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Attitude can be measured by means of electronic instrumentation on the aircraft (for example, using pitch, roll and yaw sensors), or by using a stationary tracking station which emits signals which are picked up by the aircraft and evaluated to determine its attitude. The position and attitude of an object can be determined in relation to a reference coordinate system. The reference coordinate system is defined by a plurality of reference axes, such as the North-East-Down (NED) coordinate system. In the context of an aircraft, the reference coordinate system is typically defined with respect to the surface of the earth, and can be used to establish the attitude and position of the aircraft. In order to determine the attitude and position of an object, an attitude and position measurement with respect to the reference coordinate system is required. Therefore, to determine an object's position and attitude, in practice it is necessary to provide either the position and attitude measurement with respect to the reference coordinate system or at least one of the position or attitude measurement with respect to the reference coordinate system. However, conventional methods suffer from a number of disadvantages. One such disadvantage is that, in some situations, it is not possible to measure the position and attitude of an object. For example, in the context of an aircraft, it is not possible to use either the NED coordinate system or any other conventional coordinate system on the aircraft when an aircraft is either operating in an aerodrome, or operating within the visual range of a tracking station. Another such disadvantage is that, in order to determine an object's position and/or attitude, it is necessary to provide position and/or attitude measurements with respect to the reference coordinate system. However, in some circumstances, the position and/or attitude measurements with respect to the reference coordinate system cannot be provided. For example, in the context of an aircraft it is not possible to use either the NED coordinate What's New in the? Short-term effects of the synthetic isoprostane analog, U-60,257, on neurogenesis and behavior. A synthetic isoprostane analog, U-60,257, was assessed for toxicity in the neonatal rat model. Total body weight gain, changes in the level of red blood cells, white blood cells, platelets and differential counts were assessed. Metabolic effects were assessed by evaluating changes in the levels of plasma free fatty acids, triglycerides, lactate dehydrogenase and plasma glucose and by assessing the 24-hr food consumption and the ability of rats to respond to a challenge dose of 2 mg/kg of D-amphetamine sulfate. Growth, blood cell parameters, lactate dehydrogenase and plasma glucose changes were normal in the treated animals. Methaemoglobinemia occurred in some animals. Food intake and locomotor activity were unaffected by the treatment. The effects of U-60,257 on central nervous system development were evaluated by measuring neuronal differentiation in the dentate gyrus of the hippocampus as measured by the total number of cells in the dentate gyrus, the number of cells in the subgranular zone and cells in the outer neuropil, and the number of cells with pyknotic nuclei. The number of new cells in the dentate gyrus and the number of cells in the subgranular zone were unaffected by treatment. There was a significant increase in the number of neurons in the outer neuropil. Pyknotic nuclei were only observed in the dentate gyrus of the animals treated with 600 mg/kg U-60,257. Animals treated with the dose of 1000 mg/kg had an extensive pyknosis in the dentate gyrus, with a greater than 50% reduction in the total number of pyknotic nuclei. 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